Structure and function of reconstituted AMPA receptors
نویسندگان
چکیده
Background: Ionotropic glutamate receptors mediate fast excitatory synaptic transmission in the vertebrate CNS. Results: Conformational flexibility and dimensions of functional membrane-embedded full-length GluA2flop receptors are characterized by atomic force microscopy. Conclusion: Conformational flexibility and dimensions are both strongly affected by receptor density. Significance: The reconstitution protocol lays the foundation for correlated structure-function analysis of membrane-embedded glutamate receptors.
منابع مشابه
Single channel recordings of reconstituted AMPA receptors reveal low and high conductance states.
Glutamate receptors belonging to the AMPA (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) subclass were partially purified 30- to 60-fold from forebrain of adult rats and incorporated into planar bimolecular lipid membranes. The channel conductance associated with the reconstituted receptors was activated by kainate and AMPA in a manner that suggests cooperative binding of two to thr...
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Heparin was found to bind to alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors and to alter their functional properties. AMPA receptors solubilized in 0.4% Triton X-100 bound to a heparin-agarose column and were eluted by 0.4 M NaCl. Soluble heparin inhibited 10 nM [3H]AMPA binding to detergent-solubilized receptors by 75% (IC50 = 10 micrograms/ml), but had little effect...
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